|
KMID : 0620920070390040544
|
|
Experimental & Molecular Medicine
2007 Volume.39 No. 4 p.544 ~ p.555
|
|
|
The functional role of the CARM1-SNF5 complex and its associated HMT activity in transcriptional activation by thyroid hormone receptor
|
|
Choi Hyo-Kyoung
Choi Kyung-Chul
Oh So-Young
Kang Hee-Bum
Ham Seung-Joo
Ahn Yong-Ho
Lee Yoo-Hyun
Kim Kyung-Sup
Kim Kun-Hong
Yoon Ho-Geun
|
|
|
Abstract
|
|
|
|
We have investigated the function and mechanisms of the CARM1-SNF5 complex in T3-dependent transcriptional activation. Using specific small interfering RNAs (siRNA) to knock down coactivators in HeLa α2 cells, we found that coactivator associated arginine methyltransferase 1 (CARM1) and SWI/SNF complex component 5 (SNF5) are important for T3-dependent transcriptional activation. The CARM1- SWI/SNF chromatin remodeling complex serves as a mechanism for the rapid reversal of H3-K9 methylation. Importantly, siRNA treatment against CARM1 and/or SNF5 increased the recruitment of HMTase G9a to the type 1 deiodinase (D1) promoter even with T3. Knockingdown either CARM1 or SNF5 also inhibited the downregulation of histone macroH2A, which is correlated with transcriptional activation. Finally, knocking down CARM1 and SNF5 by siRNA impaired the association of these coactivators to the D1 promoter, suggesting functional importance of CARM1- SNF5 complex in T3-dependent transcriptional activation.
|
|
|
KEYWORD
|
|
|
coactivator-associated arginine methyltransferase 1, RNA, small interfering, SMARCB1 protein, human, transcription factors
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|